Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (PE Conjugate) #14236

No. Size Price
14236S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
14236 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Pig,

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human ribosomal protein S6.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated (2 hr) with LY294002 (PI3 Kinase Inhibitor) #9901 (50 μM), Wortmannin (PI3 Kinase Inhibitor) #9951 (1 μM), and U0126 (MEK1/2 Inhibitor) #9903 (10 μM) (blue), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (PE Conjugate).

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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