Product Pathways - Apoptosis
Bak (D4E4) Rabbit mAb (PE Conjugate) #14155
|14155S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|14155||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
Bak (D4E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Bak protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Bak (D4E4) Rabbit mAb #12105.
Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
- Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
- Wei, M.C. et al. (2001) Science 292, 727-730.
- Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
- Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
- Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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