Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

PHB2 (E1Z5A) Rabbit mAb #14085

No. Size Price
14085S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
14085 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 33 Rabbit IgG
IP 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

PHB2 (E1Z5A) Rabbit mAb recognizes endogenous levels of total PHB2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHB2 protein.



Confocal immunofluorescent analysis of HT-1080 cells using PHB2 (E1Z5A) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PHB2 (E1Z5A) Rabbit mAb.



Immunoprecipitation of PHB2 from HeLa cell extracts using using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PHB2 (E1Z5A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PHB2 (E1Z5A) Rabbit mAb.


The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments (1). Prohibitin-2 (PHB2, REA) was originally identified as an estrogen receptor-specific coregulator. PHB2 directly interacts with hormone-bound estrogen receptor and represses its transcriptional activity through competitive inhibition of Src-1 coactivation of the estrogen receptor (2,3). Together with COUP transcription factors, PHB2 interacts with histone deacetylases HDAC1 and HDAC5 to mediate transcriptional regulation by the estrogen receptor through coupling the deacetylase to the transcription activation complex (4). Prohibitin PHB1/PHB2 heterodimers form large ring complexes on the mitochondrial membrane (5) and act as chaperones to stabilize mitochondrial proteins, such as OPA1 and Hax1, to support mitochondrial morphogenesis and protect against apoptosis (6-8).

  1. Mishra, S. et al. (2006) J Cell Mol Med 10, 353-63.
  2. Montano, M.M. et al. (1999) Proc Natl Acad Sci U S A 96, 6947-52.
  3. Delage-Mourroux, R. et al. (2000) J Biol Chem 275, 35848-56.
  4. Kurtev, V. et al. (2004) J Biol Chem 279, 24834-43.
  5. Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
  6. Nijtmans, L.G. et al. (2000) EMBO J 19, 2444-51.
  7. Merkwirth, C. et al. (2008) Genes Dev 22, 476-88.
  8. Kasashima, K. et al. (2006) J Biol Chem 281, 36401-10.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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