Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) #14071

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, the unconjugated antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43) by western blot analysis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with Cycloheximide #2112 (10 μg/ml, overnight) and mTNF-α #5178 (20 ng/ml, 4 hr; green), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (PE Conjugate).

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8,并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases,如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化,包括DNA的片段化和细胞固缩。

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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