Cell Signaling Technology

Product Pathways - Apoptosis

Bcl-xL (54H6) Rabbit mAb (PE Conjugate) #13835

No. Size Price
13835S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
13835 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Bcl-xL (54H6) Rabbit mAb (PE Conjugate) detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Bcl-xL (54H6) Rabbit mAb #2764.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SEM cells (blue) and K-562 cells (green) using Bcl-xL (54H6) Rabbit mAb (PE Conjugate).

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

  1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
  2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
  3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
  4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
  5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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