Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) #13797

No. Size Price
13797S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
13797 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) recognizes endogenous levels of total IL-6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse IL-6 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) #12912.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or LPS-treated (100 ng/ml, 6 hr; green), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (PE Conjugate). All cells were incubated with Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment).

Background

Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells (1,2). IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated (3). The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα (3). Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer (5).

  1. Heinrich, P.C. et al. (1998) Biochem J 334 ( Pt 2), 297-314.
  2. Heinrich, P.C. et al. (1998) Z Ernahrungswiss 37 Suppl 1, 43-9.
  3. Jones, S.A. (2005) J Immunol 175, 3463-8.
  4. Jenkins, B.J. et al. (2004) Mol Cell Biol 24, 1453-63.
  5. Hong, D.S. et al. (2007) Cancer 110, 1911-28.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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