Cell Signaling Technology

Product Pathways - NF-kB Signaling

IL-1β (D3U3E) Rabbit mAb (PE Conjugate) #13627

IL-1B  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

IL-1β (D3U3E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-1β protein and can detect up to 100 pg of recombinant mature IL-1β. This antibody is not observed to detect endogenous levels of mature IL-1β.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-1β (D3U3E) Rabbit mAb #12703.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) or LPS-treated (100 ng/ml, 3 hr; green), using IL-1β (D3U3E) Rabbit mAb (PE Conjugate).

Background

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

  1. Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99.
  2. Burns, K. et al. (1998) J Biol Chem 273, 12203-9.
  3. Cao, Z. et al. (1996) Nature 383, 443-6.
  4. Cao, Z. et al. (1996) Science 271, 1128-31.
  5. Wesche, H. et al. (1997) Immunity 7, 837-47.
  6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.
  7. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
  8. Cerretti, D.P. et al. (1992) Science 256, 97-100.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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