Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (PE Conjugate) #13498

No. Size Price
13498S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
13498 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine, Dog,

Specificity / Sensitivity

Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may cross-react with phospho-IKKε.

Source / Purification

Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by a day of rest then untreated (blue) or LPS-treated (1 μg/ml, 1 hr; green), using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (PE Conjugate).

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).

  1. Tojima, Y. et al. (2000) Nature 404, 778-82.
  2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
  3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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