Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb (PE Conjugate) #13397

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb recognizes endogenous levels of RSK1, RSK2, and RSK3 proteins only when phosphorylated at Ser380 (RSK1), Ser386 (RSK2), or Ser377 (RSK3).

Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb兔单抗能够检测内源性的磷酸化RSK1,RSK2和RSK3蛋白,磷酸化位点为Ser380 (RSK1), Ser386 (RSK2), 或 Ser377 (RSK3)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human p90RSK3 protein.

该单克隆抗体是采用合成的与人源p90RSK3蛋白的Ser377位点周围相一致的磷酸化肽段免疫动物而产生的。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb #12032.

此抗体偶联了藻红蛋白 (PE)荧光素染料,并经过了内部测试,可用于人类细胞样本的流式细胞分析检测。该抗体和未偶联的Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb #12032有相同的物种交叉反应性。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, treated with UO126 #9903 (blue) or TPA #4174 (green), using Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb (PE Conjugate).

使用Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb兔单抗 (PE Conjugate)对经过UO126 #9903 (蓝色) 或 TPA #4174 (绿色)处理的Jurkat细胞进行流式分析。

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族,其特征是包含2个不同的功能性激酶结构域(1)和一个羧基端的细胞外信号调节激酶(ERKs)结合位点(2)。RSK激酶结构域内或以外的一些位点,包括Ser380, Thr359, Ser363和Thr573,对于激酶的激活非常重要(3)。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下,通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活(3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications