Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IL-10 (D13A11) XP® Rabbit mAb (PE Conjugate) #13360

No. Size Price
13360S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
13360 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

IL-10 (D13A11) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human IL-10 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-10 (D13A11) Rabbit mAb #12163.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 5 hr), Ionomycin, Calcium Salt #9995 (2 μM, 5 hr) and Brefeldin A #9972 (3 μg/ml, last 4 hr of stimulation), using a CD3-APC antibody and IL-10 (D13A11) XP® Rabbit mAb (PE Conjugate). Analysis was performed on cells in the lymphocyte gate.

Background

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1), leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).

  1. Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
  2. Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
  3. O'Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
  4. Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
  5. Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
  6. Von Stebut, E. Eur J Dermatol 17, 115-22.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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