Product Pathways - Growth Factors/Cytokines
IL-10 (D13A11) XP® Rabbit mAb (PE Conjugate) #13360
|13360S||100 µl ( 50 tests )||￥4,264.00 现货查询||购买询价|
|13360||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
IL-10 (D13A11) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-10 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human IL-10 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-10 (D13A11) Rabbit mAb #12163.
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 5 hr), Ionomycin, Calcium Salt #9995 (2 μM, 5 hr) and Brefeldin A #9972 (3 μg/ml, last 4 hr of stimulation), using a CD3-APC antibody and IL-10 (D13A11) XP® Rabbit mAb (PE Conjugate). Analysis was performed on cells in the lymphocyte gate.
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1), leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).
- Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
- Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
- O'Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
- Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
- Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
- Von Stebut, E. Eur J Dermatol 17, 115-22.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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