Cell Signaling Technology

Product Pathways - Neuroscience

NT5E/CD73 (D7F9A) Rabbit mAb #13160

No. Size Price
13160S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13160 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 70 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

NT5E/CD73 (D7F9A) Rabbit mAb recognizes endogenous levels of total NT5E/CD73 protein.

NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗可以识别内源性的NT5E/CD73总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.

该单克隆抗体由人NT5E/CD73蛋白氨基末端重组蛋白免疫动物产生。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of BALB/c splenocytes using a CD3 antibody and Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left) or NT5E/CD73 (D7F9A) Rabbit mAb (right). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

流式细胞分析BALB/c脾细胞,使用的抗体是CD3 antibody 兔多抗和Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (左)或 NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗 (右)。Anti-rabbit IgG (H+L), F(ab')2Fragment (PE Conjugate) #8885用作二抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with cobalt chloride (CoCl2, 0.1 mM, 16 hr; +), using NT5E/CD73 (D7F9A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot分析未处理(-)或cobalt chloride处理的 (CoCl2, 0.1 mM, 16 hr; +)HeLa细胞提取物,使用的抗体是NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗(上) 或β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 and Huh7 cells, and rat eye using NT5E/CD73 (D7F9A) Rabbit mAb.

Western blot分析HT-29,Huh7细胞和大鼠眼睛组织提取物,使用的抗体是NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using NT5E/CD73 (D7F9A) Rabbit mAb.

免疫组化分析石蜡包埋的小鼠肝组织,使用的抗体是NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat kidney using NT5E/CD73 (D7F9A) Rabbit mAb.

免疫组化分析石蜡包埋的大鼠肾脏组织,使用的抗体是NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NT5E/CD73 (D7F9A) Rabbit mAb. 免疫组化分析石蜡包埋的人肺癌组织,使用的抗体是NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗。

Background

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).

Ecto-5'-nucleotidase (NT5E, 也被称为CD73)是一个70kDa的糖基磷脂酰肌醇锚定的膜结合糖蛋白,其催化细胞外单磷酸核苷水解成有生物活性的核苷(1)。NT5E催化细胞外磷酸腺苷形成腺苷,从而推动细胞外腺苷水平的调节和四个G蛋白偶联腺苷受体的下游信号激活(2)。缺氧诱导因子(HIF-1)和NT5E基因启动子结合导致缺氧时NT5E表达上调(3)。NT5E的生物功能包括林把细胞黏附(4,5),纤维化(6)和伤害感受的调节(7,8)。

  1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
  2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
  3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
  4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
  5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
  6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
  7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
  8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

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