Product Pathways - NF-kB Signaling
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate) #12914
|12914S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|12914||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine, Dog,
Specificity / Sensitivity
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate) recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346. This antibody does not react with singly phosphorylated proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr345/Ser346 of human IRAK4 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb #11927.
Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).
- Dinarello, C.A. (1996) Blood 87, 2095-147.
- Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
- Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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