Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Human MCP-1 #12900

No. Size Price
12900LC 50 µg ( With Carrier ) ¥7,763.00 现货查询 购买询价
12900LF 50 µg ( Carrier Free ) ¥7,763.00 现货查询 购买询价
12900SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
12900SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
12900 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant Human MCP-1 (hMCP-1) Gln24-Thr99 (Accession #NP_002973) was expressed in E.coli at Cell Signaling Technology.

Molecular Characterization

Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.

Bioactivity

The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED50 of each lot is between 1-11 ng/ml.

Bioactivity

Bioactivity

hMCP-1-induced migration of THP-1 cells was assessed. THP-1 cells were incubated in a 96-well transwell plate with increasing concentrations of hMCP-1 in the bottom chamber. After 2 hr, the number of THP-1 cells that migrated to the bottom chamber of the transwell was quantified by measuring DNA content using a fluorescent dye.

Coomassie Gel

Coomassie Gel

The purity of recombinant hMCP-1 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hMCP-1 and staining overnight with Coomassie Blue.

Applications

Optimal concentration for the desired application should be determined by the user.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hMCP-1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hMCP-1. Carrier free: Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2.

Background

MCP-1 (CCL2) is the first member of the C-C family of chemokines to be identified (1). C-C chemokines are characterized by two adjacent cysteine residues within the polypeptide, which form an intra-molecular disulfide bond. MCP-1 is a potent chemotactic factor for monocytes/macrophages, T cells and a subset of NK cells (1-4). The MCP-1 receptor, CCR2, is expressed as two splice isoforms, CCR2A and CCR2B, of which CCR2B is the predominant form (1). MCP-1 is secreted by adipocytes and appears to be one of many links between obesity, inflammation, and diabetes (1). MCP-1/CCR2 signaling appears to play a key role in γδ effector T cells recruitment and anti-tumor responses in a murine B16 melanoma model (2). Conversely, CCL2 expression is upregulated in many types of cancer and has been implicated in promoting tumor cell survival, proliferation, and tumor associated inflammation (4).

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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