Product Pathways - Growth Factors/Cytokines
IL-4 (D19A10) Rabbit mAb (PE Conjugate) #12884
|12884S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|12884||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
IL-4 (D19A10) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-4 (D19A10) Rabbit mAb #12227.
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 5 hr), Ionomycin, Calcium Salt #9995 (2 μM, 5 hr), and Brefeldin A #9972 (3 μg/ml, last 4 hr of stimulation), using a CD3-APC antibody and IL-4 (D19A10) Rabbit mAb (PE Conjugate). Analysis was performed on cells in the lymphocyte gate.
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
- Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Kopf, M. et al. (1993) Nature 362, 245-8.
- Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
- Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
- Hou, J. et al. (1994) Science 265, 1701-6.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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