Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IL-4 (D19A10) Rabbit mAb (PE Conjugate) #12884

IL4  

No. Size Price
12884S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
12884 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

IL-4 (D19A10) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-4 (D19A10) Rabbit mAb #12227.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 5 hr), Ionomycin, Calcium Salt #9995 (2 μM, 5 hr), and Brefeldin A #9972 (3 μg/ml, last 4 hr of stimulation), using a CD3-APC antibody and IL-4 (D19A10) Rabbit mAb (PE Conjugate). Analysis was performed on cells in the lymphocyte gate.

Background

Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).

  1. Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
  2. Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
  3. Kopf, M. et al. (1993) Nature 362, 245-8.
  4. Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
  5. Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
  6. Hou, J. et al. (1994) Science 265, 1701-6.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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