Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

FGF Receptor 1 (D8E4) XP® Rabbit mAb (PE Conjugate) #12777

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

FGF Receptor 1 (D8E4) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total FGF receptor 1 protein. This antibody does not cross-react with other FGF receptor family members.

FGF Receptor 1 (D8E4) XP® 兔单抗 (PE标记)能够检测内源性的总FGF受体蛋白1的水平,该抗体并不会与其它FGF受体家族成员产生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.

该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白是人FGF受体1蛋白的羧基末端。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740.

该Cell Signaling Technology抗体与藻红蛋白(PE)结合,内部测试用人细胞进行直标流式细胞分析。该抗体与未结合的FGF Receptor 1 (D8E4) XP® 兔单抗#9740预期表现出同一物种的交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-29 cells (blue) and A-204 cells (green) using FGF Receptor 1 (D8E4) XP® Rabbit mAb (PE Conjugate). 流式细胞术方法检测HT-29细胞(蓝色)和A-204细胞(绿色),使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (PE Conjugate).

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).for downstream signaling components such as Crk and PLCγ (4,5).

成纤维细胞生长因子 (FGFs)通过细胞表面的酪氨酸激酶受体传递信号,对靶细胞产生促有丝分裂和血管生成的效应。已知FGF受体家族有四个成员:FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3和FGFR-4。每个受体都有一个胞外配体结合区、跨膜区和一个胞内激酶区(1)。在结合配体和二聚化之后,受体的特定的酪氨酸位点发生磷酸化(2)。在FGFR-1的羧基端有7个酪氨酸位点能被磷酸化:Tyr463, 583, 585, 653, 654, 730和 766。Tyr653 和Tyr654对激活的FGFR的催化活性十分重要,对信号传导也是必要的(3)。其它的磷酸化酪氨酸位点能够为下游信号分子,如Crk 和PLCγ,提供结合位点(4,5)。

  1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
  2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
  3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
  4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
  5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications