Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-1 (D5E4) XP® Rabbit mAb (PE Conjugate) #12732

No. Size Price
12732S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
12732 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

IRF-1 (D5E4) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IRF-1 protein.

IRF-1 (D5E4) XP® Rabbit mAb (PE偶联) 能够检测内源性IRF-1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro261 of human IRF-1 protein.

此单克隆抗体通过合成肽免疫动物制备,这种合成肽是人源IRF-1蛋白Pro261周围肽段。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IRF-1 (D5E4) XP® Rabbit mAb #8478.

这种Cell Signaling Technology的抗体能够与藻红蛋白(PE)相结合,并通过人源细胞的流式细胞分析来进行内部检测。此抗体与未共轭的IRF-1 (D5E4) XP® Rabbit mAb #8478一样,具有相同物种之间的交叉反应性。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Human Interferon-γ (hIFN-γ) #8901 (green), using IRF-1 (D5E4) XP® Rabbit mAb (PE Conjugate).流式细胞术分析HeLa细胞,未处理(蓝色)或Human Interferon-γ (hIFN-γ) #8901处理(绿色),使用抗体是IRF-1 (D5E4) XP® Rabbit mAb (PE Conjugate)。

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).

干扰素调控因子(IRFs) 包含一个家族的转录调控因子,这个家族主要是在 Jak/Stat信号通路中起作用,在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自体免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1、IRF-2、ISGF3γ/p48、IRF-3、 IRF-4 (Pip/LSIRF/ICSAT)、 IRF-5、 IRF-6、 IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都具有同源性。IRF家族成员通过与具有相似DNA-结合区域(例如IFN刺激的应答原件(ISRE)、IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E)) 的蛋白相互作用而调控转录(2)。

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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