Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

MouseHis6Interleukin-21 (mHis6IL-21) #12670

No. Size Price
12670LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
12670LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
12670SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
12670SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
12670 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse His6IL-21 (mHis6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant His6-tagged IL-21 has a calculated MW of 16,952. DTT-reduced and non-reduced protein migrate as 18-28 kDa polypeptides. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino terminus of recombinant mHis6IL-21 was verified by amino acid sequencing.


>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-21. All lots are greater than 95% pure.


The bioactivity of recombinant mHis6IL-21 was determined in a B9 cell proliferation assay. The ED50 of each lot is between and 20-80 pg/ml.



The proliferation of B9 cells treated with increasing concentrations of mHis6IL-21 was assessed. After a 72 hour treatment with mHis6IL-21, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Coomassie Gel

Coomassie Gel

The purity of recombinant mHis6 IL-21 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6 IL-21 and staining overnight with Coomassie Blue.


Optimal concentration for the desired application should be determined by the user.


Less than 0.01 ng endotoxin/1 μg mHis6IL-21.


With carrier: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution containing 20 μg BSA per 1 μg mHis6IL-21. Carrier free: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution.


IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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