渲染靶标：SSR
Render Timestamp:
4/4/2025, 5:05:35 AM EDT
4/4/2025, 9:05:35 AM UTC
Commit: 070ddbed3d58ff00053de84939cefdd328876779
XML generation date: 2025-03-07 13:06:29.511
Product last modified at: 2024-05-30T07:10:10.567Z
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PDP - Template Name: siRNA
PDP - Template ID: *******aa36529
  1. Products /
  2. siRNA /
  3. SignalSilence® FLIP siRNA II

SignalSilence® FLIP siRNA II #12407

SignalSilence® FLIP siRNA II: Image 1
Western blot analysis of extracts from A549 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® FLIP siRNA I #12372 (+), or SignalSilence® FLIP siRNA II (+), using FLIP (D16A8) Rabbit mAb #8510 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The FLIP (D16A8) Rabbit mAb confirms silencing of FLIP expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
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Supporting Data

REACTIVITY H
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

CST recommends transfection with 100 nM SignalSilence® FLIP siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Storage

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® FLIP siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit FLIP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Background

Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin's lymphoma and ovarian and colon carcinomas (9).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: O15519


Entrez-Gene Id: 8837


限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

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For Research Use Only. Not For Use In Diagnostic Procedures.
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SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
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