Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IFN-γ (D3H2) XP® Rabbit mAb (PE Conjugate) #12274

No. Size Price
12274S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
12274 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

IFN-γ (D3H2) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IFN-γ protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IFN-γ protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IFN-γ (D3H2) XP® Rabbit mAb #8455.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 4 hr); Ionomycin, Calcium Salt #9995 (2 μM, 4 hr); and Brefeldin A #9972 (1 μg/ml, last 3 hr of stimulation), using a CD3 antibody and IFN-γ (D3H2) XP® Rabbit mAb (PE Conjugate). Analysis was performed on cells in the lymphocyte gate

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

  1. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
  2. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
  3. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
  4. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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