Product Pathways - Cell Cycle / Checkpoint
Cyclin D1 (92G2) Rabbit mAb (PE Conjugate) #12205
|12205S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|12205||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
Cyclin D1 (92G2) Rabbit mAb (PE Conjugate) detects endogenous levels of total cyclin D1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human cyclin D1.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cyclin D1 (92G2) Rabbit mAb #2978.
Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
- Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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