Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

p27 Kip1 (D69C12) XP® Rabbit mAb (PE Conjugate) #12184

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

p27 Kip1 (D69C12) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total p27 Kip1 protein.p27 Kip1 (D69C12) XP® Rabbit mAb (PE 偶联)可以识别内源性p27 Kip1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p27 Kip1 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人p27 Kip1蛋白的氨基端邻近残基序列一致。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated p27 Kip1 (D69C12) XP® Rabbit mAb #3686.Cell Signaling Technology公司抗体偶联藻红蛋白(PE)并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的p27 Kip1 (D69C12) XP®)Rabbit mAb #3686显示同样的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP® Rabbit mAb (PE Conjugate) and DRAQ5® #4084 (fluorescent DNA dye).使用p27 Kip1 (D69C12) XP® Rabbit mAb (PE 偶联)和DRAQ5® #4084 (fluorescent DNA dye)对Jurkat细胞进行流式分析。

Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).p27 Kip1是细胞周期蛋白激酶抑制因子Cip/Kip家族的一员。与它的同类蛋白类似,p57 Kip2 和p21 Waf1/Cip1,它实施G1限制点的能力来自于阻止和CDK2/cyclin E和其他CDK/cyclin复合物结合。p27的表达水平在休眠细胞和被cAMP或其他细胞周期负调因子处理过的细胞中表达上调。白介素2或其他有丝分裂原处理后能够下调p27;这一机制设计了p27的磷酸化,p27的降解是通过泛素-蛋白酶体通路进行的(1-4)。

  1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
  2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
  3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
  4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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