Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ki-67 (D3B5) Rabbit mAb (PE Conjugate) #12160

MIB1   SP6  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Ki-67 protein.Ki-67 (D3B5)Rabbit mAb (PE 偶联)可以识别内源性Ki-67总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human Ki-67 protein. 单克隆抗体由合成肽段免疫动物产生,该肽段与人Ki-67蛋白的氨基端邻近残基序列一致。


This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.Cell Signaling Technology公司抗体偶联藻红蛋白(PE)并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (PE Conjugate) and DRAQ5® #4084 (fluorescent DNA dye).使用Ki-67 (D3B5)Rabbit mAb (PE 偶联)和DRAQ5® #4084 (fluorescent DNA dye)对Jurkat细胞进行流式分析。


Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).Ki-67,以其发现地命名(Kiel大学,德国),作为一个细胞核非组蛋白(1)通常在增殖的细胞中表达并且在休眠细胞中不表达(2)。Ki-67会让增殖细胞停滞在G1,S,G2和有丝分裂期,但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关(3)。研究已经将Ki-67与其他的标记,作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记(4)。

  1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
  3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
  4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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