Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate) #12020

VIM  

No. Size Price
12020S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
12020 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total vimentin protein.

Vimentin (D21H3) XP® Rabbit mAb(偶联PE)可以识别内源性总的Vimentin 蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人vimentin的Arg45邻近残基序列一致。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Vimentin (D21H3) XP® Rabbit mAb #5741.

Cell Signaling Technology公司抗体偶联藻红蛋白(PE)并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Vimentin (D21H3) XP® Rabbit mAb #5741显示同样的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate).使用Vimentin (D21H3) XP® Rabbit mAb(偶联PE)对MCF7(蓝色)和HeLa(绿色)进行流式分析。

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).

细胞骨架由三种细胞纤维构成:微丝(肌动蛋白丝),中间丝和微管。中间丝的主要类型由它们的细胞特异性表达来区分:细胞角蛋白(上皮细胞),胶质原纤维酸性蛋白(GFAP)(神经胶质细胞),结蛋白(desmin)(骨骼,内脏,某些血管平滑肌细胞),波形蛋白(间充质起源),神经丝蛋白(神经元)。星形胶质细胞中GFAP和vimentin形成中间丝以调节它们的运动和形状(1)。尤其是vimentin中间丝出现在发育早期,GFAP中间丝是终末分化的成熟脑星形胶质细胞的标记(2)。研究表明vimentin在肉瘤中表达,而在肿瘤中不表达,它的表达与其他标记蛋白相结合用于研究区分这二者(3)。Vimentin的多变的结构和空间重组特性是对细胞外刺激的应激,帮助细胞协同调节多种信号通路(4)。肌肉细胞中Vimentin的56位丝氨酸磷酸化会调节vimentin中间丝的结构以应对肉瘤(5,6)。Vimentin和其他中间丝的重构对于淋巴细胞黏附及通过内皮细胞的迁移是非常重要的(7)。

  1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
  3. Leader, M. et al. (1987) Histopathology 11, 63-72.
  4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
  5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
  6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
  7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
  8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
  9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
  10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
  11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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