Product Pathways - Growth Factors/Cytokines
Human TL1A/TNFSF15 (hTL1A) #11946
|11946LC||50 µg ( With Carrier )||￥5,949.00 现货查询||购买询价|
|11946LF||50 µg ( Carrier Free )||￥5,949.00 现货查询||购买询价|
|11946SC||10 µg ( With Carrier )||￥2,061.00 现货查询||购买询价|
|11946SF||10 µg ( Carrier Free )||￥2,061.00 现货查询||购买询价|
|11946||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant Human TL1A Leu72-Leu251 (Accession #NP_095150) was expressed in E.coli at Cell Signaling Technology.
重组人TL1A Leu72-Leu251 (Accession #NP_095150)由CST公司在大肠杆菌中进行表达。
Recombinant hTL1A has a calculated MW of 20,047Da. DTT-reduced protein migrates as a 21 kDa polypeptide. The nonreduced protein migrates as a 21 kDa monomer and 38 kDa cystine-linked homodimer. The expected amino terminus of recombinant hTL1A was verified by amino acid sequencing.
>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hTL1A. All lots are greater than 95% pure.
The bioactivity of hTL1A was determined in a TF-1 cell viability assay. The ED50 of each lot is between 2-15 ng/ml.
The viability of TF-1 cells treated with increasing amounts of hTL1A in the presence of 10 µg/ml cyclohexamide was determined. After a 24 hr treatment with hTL1A, cells were incubated with tetrazolium salt and the OD450 - OD650 was determined.通过不断增加含10 µg/ml环己酰胺的hTL1A的量来测定TF-1细胞的生存力。用hTL1A作用24小时后，细胞中加入四唑盐继续培养，并测定OD450-OD650。
Less than 0.01 ng endotoxin/1 μg hTL1A.
With carrier: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hTL1A. Carrier free: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2.
有载体：将hTL1A溶液（20mM Tris，pH7.2，每1 μg hTL1A中含20 μg BSA）经0.22μm滤膜过滤并冻干。
TL1A (TNFSF15), a member of the TNF superfamily of proteins, is a splice variant of the TL1/VEGI gene (1). Endothelial cells, monocytes, macrophages, and dendritic cells express TL1A, which is upregulated by proinflammatory cytokines, microorganisms, and AMPK activation (1-4). TL1A activates the NF-κB and JNK pathways through its receptor, DR3 (1,5). TL1A may function as a costimulatory signal for T cell activation, specifically regulating Th17 cell development and proliferation (1,2,6). Mouse models suggest a role for TL1A as a driver for the inflammation and pathogenesis associated with inflammatory bowel disease (7,8).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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