Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human TL1A/TNFSF15 (hTL1A) #11946

TNFSF15  

No. Size Price
11946LC 50 µg ( With Carrier ) ¥5,949.00 现货查询 购买询价
11946LF 50 µg ( Carrier Free ) ¥5,949.00 现货查询 购买询价
11946SC 10 µg ( With Carrier ) ¥2,061.00 现货查询 购买询价
11946SF 10 µg ( Carrier Free ) ¥2,061.00 现货查询 购买询价
11946 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant Human TL1A Leu72-Leu251 (Accession #NP_095150) was expressed in E.coli at Cell Signaling Technology. 

重组人TL1A Leu72-Leu251 (Accession #NP_095150)由CST公司在大肠杆菌中进行表达。

Molecular Characterization

Recombinant hTL1A has a calculated MW of 20,047Da. DTT-reduced protein migrates as a 21 kDa polypeptide. The nonreduced protein migrates as a 21 kDa monomer and 38 kDa cystine-linked homodimer. The expected amino terminus of recombinant hTL1A was verified by amino acid sequencing.

重组hTL1A的分子量为20,047Da。DTT还原蛋白接近21 kDa的多肽的迁移位置。非还原蛋白分为21kDa的单体和38kDa的胱氨酸联同型二聚体。重组hTL1A预期的氨基末端通过氨基酸测序进行了验证。

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hTL1A. All lots are greater than 95% pure.

用6 μg还原的(+)和非还原的(-)重组hTL1A进行SDS-PAGE,纯度都大于95%。所有批次的产品纯度都大于95%。

Bioactivity

The bioactivity of hTL1A was determined in a TF-1 cell viability assay. The ED50 of each lot is between 2-15 ng/ml. 

用TF-1细胞生存试验进行hTL1A的生物活性测定。每个批次的ED50在2-15 ng/ml之间。

Bioactivity

Bioactivity

The viability of TF-1 cells treated with increasing amounts of hTL1A in the presence of 10 µg/ml cyclohexamide was determined. After a 24 hr treatment with hTL1A, cells were incubated with tetrazolium salt and the OD450 - OD650 was determined.通过不断增加含10 µg/ml环己酰胺的hTL1A的量来测定TF-1细胞的生存力。用hTL1A作用24小时后,细胞中加入四唑盐继续培养,并测定OD450-OD650。

Coomassie Gel

Coomassie Gel

The purity of recombinant hTL1A was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hTL1A and staining overnight with Coomassie Blue.重组hTL1A的纯度通过SDS-PAGE进行测定。用6 µg还原的(+)和非还原的(-)重组hTL1A进行SDS-PAGE,考马斯亮蓝染色过夜。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hTL1A.

每微克hTL1A内毒素含量小于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hTL1A. Carrier free: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2.

有载体:将hTL1A溶液(20mM Tris,pH7.2,每1 μg hTL1A中含20 μg BSA)经0.22μm滤膜过滤并冻干。

无载体:将hTL1A溶液(20mM Tris,pH7.2)经0.22μm滤膜过滤并冻干。

Background

TL1A (TNFSF15), a member of the TNF superfamily of proteins, is a splice variant of the TL1/VEGI gene (1). Endothelial cells, monocytes, macrophages, and dendritic cells express TL1A, which is upregulated by proinflammatory cytokines, microorganisms, and AMPK activation (1-4). TL1A activates the NF-κB and JNK pathways through its receptor, DR3 (1,5). TL1A may function as a costimulatory signal for T cell activation, specifically regulating Th17 cell development and proliferation (1,2,6). Mouse models suggest a role for TL1A as a driver for the inflammation and pathogenesis associated with inflammatory bowel disease (7,8).

TL1A (TNFSF15)是肿瘤坏死因子超家族成员之一,是TL1/VEGI基因的剪接变体(1)。内皮细胞、单核细胞、巨噬细胞和树突细胞都能表达TL1A,TL1A在促炎细胞因子、微生物和AMPK活化的作用下能上调(1-4)。TL1A通过其受体DR3能活化NF-κB和JNK通路(1,5)。TL1A可能作为T细胞活化的协同刺激信号,尤其是调节Th17细胞发育和增殖(1,2,6)。小鼠模型显示TL1A是炎性肠病相关炎症和发病机制的“驱动器”(7,8)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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