Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate) #11917

Rb  

No. Size Price
11917S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
11917 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb (PE偶联)可以识别807和811位丝氨酸分别磷酸化或同时磷酸化后的内源性Rb蛋白。抗体与608位丝氨酸磷酸化后的Rb蛋白没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人Rb蛋白的807/811位丝氨酸邻近残基序列一致。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.Cell Signaling Technology公司抗体偶联藻红蛋白(PE)并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb #8516显示同样的物种交叉反应.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate).使用Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb (PE偶联)对BT-549细胞(蓝色)和Jurkat细胞(绿色)进行流式细胞分析。

Background

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).成视网膜细胞瘤抑制蛋白,Rb,通过控制G1期的细胞限制点以调控细胞增殖(1)。Rb有3个功能迥异的结构域,与一系列重要的调控蛋白相结合,包括E2F家族调控因子,c-Abl酪氨酸激酶以及带有LXCXE保守结构域的蛋白(2-4)。CDK引起的细胞周期依赖性磷酸化会抑制Rb与靶蛋白结合从而促进细胞周期的进行(5)。细胞周期蛋白 D-CDK4/6起始的磷酸化,伴随着细胞周期蛋白E-CDK2的磷酸化,似乎能够失活Rb并引发细胞周期(6)。体外实验已经检测到有不同的细胞周期蛋白依赖性激酶/细胞周期蛋白复合物(6-8)同时体内试验证明细胞周期蛋白D1对于780位丝氨酸的磷酸化是必需的(9)。

  1. Sherr, C.J. (1996) Science 274, 1672-7.
  2. Nevins, J.R. (1992) Science 258, 424-9.
  3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
  4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
  5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
  6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
  8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
  9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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