Cell Signaling Technology

Product Pathways - Metabolism

MDH2 (D8Q5S) Rabbit mAb #11908

M-MDH   malate dehydrogenase 2. NAD (mitochondrial)   Malate dehydrogenase. mitochondrial   MDH   MDH2   MDHM   MGC:3559   MOR1  

No. Size Price
11908S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11908 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 35 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

MDH2 (D8Q5S) Rabbit mAb recognizes endogenous levels of total MDH2 protein.

MDH2 (D8Q5S) Rabbit mAb能够检测内源性MDH2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly306 of human MDH2 protein.

该单克隆抗体是由合成的人源MDH2蛋白甘氨酸(306位)肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MDH2 (D8Q5S) Rabbit mAb.

Wetern blot检测多种细胞系提取物,所用抗体为MDH2 (D8Q5S) Rabbit mAb兔单抗。

Background

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate/aspartate shuttle (1-3). The major function of the mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2).

苹果酸脱氢酶(MDH)是三羧酸循环和苹果酸/天冬氨酸穿梭过程中的关键酶(1,2)。MDH广泛表达于大多数细菌和所有的真核生物 (2)。细胞质MDH同工酶(cMDH或MDH1)主要是在苹果酸/天冬氨酸穿梭过程中将草酰乙酸还原成苹果酸(1,2,3)。而线粒体MDH同工酶(mMDH或MDH2)的作用主要是在三羧酸循环中将苹果酸氧化为草酰乙酸(1,2)。

  1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
  2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
  3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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