Cell Signaling Technology

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PAI-1 (D9C4) Rabbit mAb #11907

No. Size Price
11907S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
11907 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey,Bovine, Endogenous 48 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

PAI-1 (D9C4) Rabbit mAb recognizes endogenous levels of total PAI-1 protein.

PAI-1 (D9C4) Rabbit mAb用于检测内源性的PAI-1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg294 of human PAI-1 protein.

该单克隆抗体由人PAI-1蛋白Arg294 附近残基合成的多肽免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 and HUVEC cells using PAI-1 (D9C4) Rabbit mAb.Western blot分析COS-7和HUVEC细胞提取物,采用的抗体是PAI-1 (D9C4) Rabbit mAb。



Immunoprecipitation of PAI-1 from COS-7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or PAI-1 (D9C4) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PAI-1 (D9C4) Rabbit mAb. 免疫沉淀分析 COS-7细胞提取物,采用的抗体是Normal Rabbit IgG #2729 (lane 2) or PAI-1 (D9C4) Rabbit mAb (lane 3)。Lane 1为10%的Input。Western blot分析采用的抗体是PAI-1 (D9C4) Rabbit mAb。


PAI-1 is a secreted protein that belongs to the serine proteinase inhibitor (serpin) superfamily. It inhibits urokinase and tissue plasminogen activators (uPA and tPA) and thus, reduces the conversion of inactive plasminogen to plasmin (1). PAI-1 regulates fibrinolysis and plays an important role in vessel patency and tissue remodeling. Secreted PAI-1 interacts with the extracellular matrix (ECM) component vitronectin, thereby modulating cell-ECM interactions (2,3). PAI-1 is expressed in a variety of tissues with higher expression in liver, vascular endothelial cells, platelets, macrophages, and adipose tissue (1). Increased levels of PAI-1 are associated with deep vein thrombosis (4). Defects in PAI-1 cause plasminogen activator inhibitor-1 deficiency (PAI-1D), which is characterized by increased bleeding after injury or surgery (5). Research studies have shown that high levels of PAI-1 are associated with obesity, aging, insulin resistance, and type 2 diabetes (6-8). PAI-1 is transcriptionally regulated by TGF-β and mediates TGF-β-induced inhibition of cell migration and invasion in cancer cells (9). Studies have shown PAI-1 to be also involved in fibrosis (10).

PAI-1是一种分泌性蛋白,属于丝氨酸蛋白酶抑制超家族。它可以抑制尿激酶和组织纤维蛋白溶酶原激活剂(uPA 和tPA),因此减少了无活性的纤溶酶原向纤溶酶的转化(1)。PAI-1调节纤维蛋白溶解作用,并在血管开放和组织重建中起重要作用。分泌型的PAI-1与细胞外基质组分玻璃粘连蛋白相互作用,从而调节细胞-细胞外基质相互作用(2,3)。PAI-1在多种组织表达,在肝脏、血管内皮细胞、血小板、巨噬细胞和脂肪组织高表达(1)。PAI-1的表达增高与深部静脉血栓形成有关。PAI-1的缺乏可以引起纤溶酶原活化因子抑制因子-1缺乏(PAI-1D),该抑制因子可以促进受伤或手术后出血(5)。研究表明高水平的PAI-1与肥胖、衰老、胰岛素抵抗和2型糖尿病有关(6-8)。PAI-1的转录可以被TGF-β调节,并介导TGF-β对细胞迁移和癌症细胞浸润的抑制(9)。研究表明PAI-1在纤维化中也起作用(10)。

  1. Pannekoek, H. et al. (1986) EMBO J 5, 2539-44.
  2. Sigurdardottir, O. and Wiman, B. (1994) Biochim Biophys Acta 1208, 104-10.
  3. Konstantinides, S. et al. (2001) Circulation 103, 576-83.
  4. Baldwin, J.F. et al. (2012) J Vasc Surg 56, 1089-97.
  5. Fay, W.P. et al. (1997) Blood 90, 204-8.
  6. Pannacciulli, N. et al. (2002) Obes Res 10, 717-25.
  7. Juhan-Vague, I. et al. (1991) Diabetologia 34, 457-62.
  8. Hashimoto, Y. et al. (1987) Thromb Res 46, 625-33.
  9. Humbert, L. and Lebrun, J.J. (2012) Cell Signal , .
  10. Zhang, L.P. et al. (1999) J Hepatol 31, 703-11.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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