Cell Signaling Technology

Product Pathways - DNA Damage

WIP1 (D4F7) Rabbit mAb #11901

No. Size Price
11901S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
11901 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 79 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

WIP1 (D4F7) Rabbit mAb recognizes endogenous levels of total WIP1 protein.WIP1 (D4F7) Rabbit mAb可以识别内源性WIP1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly240 of human WIP1 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人WIP1蛋白的240位甘氨酸邻近残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WIP1 (D4F7) Rabbit mAb.使用WIP1 (D4F7) Rabbit mAb对多种细胞提取物进行western blot。

Background

Wild-type p53 induced phosphatase 1 (WIP1)/protein phosphatase magnesium-dependent 1 delta (ppm1d) is a member of the PP2C family of serine/threonine protein phosphatases. WIP1 was initially identified as a p53 target gene, induced in response to ionizing radiation (1). Studies have shown that WIP1 is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (reviewed in 2,3). WIP1 functions in returning cells to a homeostatic state following DNA damage (4,5), as well as in maintaining p53-dependent homeostasis under nonstress conditions (6). Researchers have shown that increased expression of WIP1 is associated with poor prognosis and lower survival rate in some human cancers (7,8). In contrast, overexpression of WIP1 in p53-negative tumor cells sensitizes them to chemotherapy-induced apoptosis while protecting normal tissue during treatment (9).使用WIP1 (D4F7) 兔mAb对多种细胞提取物进行western blot。野生型p53诱导磷酸酶1(WIP1)/蛋白磷酸酶镁依赖1delta(ppm1d)是丝氨酸/苏氨酸蛋白磷酸酶家族PP2C的一员。WIP1最初被认为是电离辐射应激后p53的靶基因(1)。研究证明WIP1在人类癌症中过表达并设计到调控多种DNA损伤信号通路(2,3)。WIP1发挥着在DNA损伤后将细胞送回稳定状态的功能(4,5),同时也维持着非压力条件下p53-依赖的稳态(6)。研究人员发现WIP1的过表达与某些人类肿瘤的不良预后和低存活率相关(7,8)。相对的,在p53缺乏的肿瘤细胞中过表达WIP1能够在治疗过程中加强细胞对于化疗诱导的凋亡的敏感性以通过保护正常组织(9)。

  1. Fiscella, M. et al. (1997) Proc Natl Acad Sci U S A 94, 6048-53.
  2. Zhu, Y.H. and Bulavin, D.V. (2012) Prog Mol Biol Transl Sci 106, 307-25.
  3. Lu, X. et al. (2008) Cancer Metastasis Rev 27, 123-35.
  4. Lu, X. et al. (2005) Genes Dev 19, 1162-74.
  5. Cha, H. et al. (2010) Cancer Res 70, 4112-22.
  6. Park, H.K. et al. (2011) Cell Cycle 10, 2574-82.
  7. Liang, C. et al. (2012) Brain Res 1444, 65-75.
  8. Satoh, N. et al. (2011) Cancer Sci 102, 1101-6.
  9. Goloudina, A.R. et al. (2012) Proc Natl Acad Sci U S A 109, E68-75.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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