Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD8 (D3C1) Rabbit mAb #11891

CHD   CHD-8  

No. Size Price
11891S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
11891 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 290 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CHD8 (D3C1) Rabbit mAb recognizes endogenous levels of total CHD8 protein. This antibody also cross-reacts with a protein of unknown origin at 140 kDa.

CHD8 (D3C1) Rabbit mAb兔单抗能够检测内源性CHD8总蛋白水平。该抗体还能与一个140kDa的未知来源蛋白产生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CHD8 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8 and SR cells using CHD8 (D3C1) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为CHD8 (D3C1) Rabbit mAb。



Immunoprecipitation of CHD8 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD8 (D3C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD8 (D3C1) Rabbit mAb.从 SH-SY5Y细胞提取物中免疫沉淀CHD8,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或CHD8 (D3C1) Rabbit mAb (lane 3)。Lane 1为10% input。 使用CHD8 (D3C1) Rabbit mAb进行Western blot检测。


CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10).

CHD8属于ATP依赖的染色质重塑蛋白的chromodomain helicase DNA-binding (CHD)家族 (1)。研究证明CHD蛋白家族通过利用ATP水解的能量去改变染色质架构从而在基因表达调控中起到重要作用(1,2)。九种CHD家族成员都有一个特点,即在N-端区域有两个串联的染色质域而在蛋白中间区域有一个SNF2-like ATPase结构域(2-4)。此外,CHD8还包含三个CR (conserved region)结构域、一个SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like结构域、两个BRK (brahma and kismet)结构域以及一个DNA结合结构域 (2)。研究显示CHD8蛋白的染色质重塑活性对于很多基因调控是非常重要的,例如HOX基因(5)以及一些由β-连环蛋白 (6)、p53 (7)、雌激素受体 (8)或雄激素受体 (9)驱动的基因。CHD8蛋白还能够与绝缘子结合蛋白质CTCF相互作用,并且是CTCF在多种基因座的绝缘子活性所需要的(10)。

  1. Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8.
  4. Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7.
  5. Yates, J.A. et al. (2010) FEBS Lett 584, 689-93.
  6. Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
  7. Nishiyama, M. et al. (2009) Nat Cell Biol 11, 172-82.
  8. Caldon, C.E. et al. (2009) Mol Cell Biol 29, 4623-39.
  9. Menon, T. et al. (2010) Mol Endocrinol 24, 1165-74.
  10. Ishihara, K. et al. (2006) Mol Cell 23, 733-42.

Application References

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