Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat, Endogenous Rabbit IgG
IF-F 1:200
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.Ki-67 (D3B5) Rabbit mAb (偶联Alexa Fluor® 488)可以识别内源性总的Ki-67蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Ki-67 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人Ki-67蛋白的氨基端一致。


This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.Cell Signaling Technology公司抗体偶联Alexa Fluor® 488荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应.



Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).使用Ki-67 (D3B5) Rabbit mAb (偶联Alexa Fluor® 488) (绿色)对HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记(红色)。



Confocal immunofluorescent analysis of normal rat colon using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Ki-67 (D3B5) Rabbit mAb (偶联Alexa Fluor® 488) (绿色)对正常大鼠结肠组织进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) and Propidium Iodide (PI) /RNase Staining Solution #4087 (DNA content).使用Ki-67 (D3B5) Rabbit mAb (偶联Alexa Fluor® 488)和碘化丙啶(PI) /RNase固定液#4087(DNA content)对Jurkat细胞进行流式分析。


Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).Ki-67,以其发现地命名(Kiel大学,德国),作为一个细胞核非组蛋白(1)通常在增殖的细胞中表达并且在休眠细胞中不表达(2)。Ki-67会让增殖细胞停滞在G1,S,G2和有丝分裂期,但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关(3)。研究已经将Ki-67与其他的标记,作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记(4)。

  1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
  3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
  4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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