Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) #11880

p75  

No. Size Price
11880S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
11880 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE偶联)兔单抗只能检测Tyr397位点发生磷酸化时的内源性HS1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

此单克隆抗体通过合成鼠源HS1蛋白Tyr405位点周围的肽段免疫动物制备。这个位点与人源HS1蛋白的Tyr397相对应。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714.

这种Cell Signaling Technology的抗体与藻红蛋白(PE)相偶联,并且通过对人源细胞的流式细胞分析来进行内部检测。此抗体与未偶联的Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714一样,具有同物种之间的交叉反应性。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate). 流式细胞术分析Ramos细胞,未处理(蓝色)或anti-IgM和过氧化氢处理(绿色),使用抗体是 Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate)。

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

HS1 (HCLS1, LckBP1, p75)是一种蛋白激酶底物,它只在具有造血功能的组织和细胞中表达(1,2)。HS1 包含了四个皮肌动蛋白重复和一个单独的SH3 域(2)。在免疫受体的激活后,胞内蛋白被磷酸化,从而促进了HS1被招募到免疫突触处(3-5)。HS1的磷酸化是调控肌动蛋白动力不可缺少的,同时也为其他的信号分子如Vav1和 PLCγ1 提供了结合位点(6)。HS1也在血小板激活中发挥了重要的作用(7)。

HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

在免疫受体刺激和凝血酶介导的血小板刺激下,HS1能够快速的被Syk和/或 Lyn激酶在Tyr397位点处磷酸化. 此磷酸化是细胞骨架重排和信号复合体形成过程中重要的一步(6-10)。

  1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
  2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
  4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
  5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
  6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
  7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
  8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
  9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
  10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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