Cell Signaling Technology

Product Pathways - Protein Translation

Upf2 (D3B10) Rabbit mAb #11875

DKFZp434D222   hUpf2   KIAA1408   MGC138834   MGC138835   Nonsense mRNA reducing factor 2   Regulator of nonsense transcripts 2   RENT2   smg-3   Up-frameshift suppressor 2 homolog   UPF2   UPF2 regulator of nonsense transcripts homolog   UPF2 regulator of nonsense transcripts homolog (yeast)   yeast Upf2p homolog  

No. Size Price
11875S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
11875 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 170 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Upf2 (D3B10) Rabbit mAb recognizes endogenous levels of total Upf2 protein.

Upf2 (D3B10) Rabbit mAb兔单抗能够检测内源性的Upf2总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Upf2 protein.

该单克隆抗体是经由合成的围绕人Upf2蛋白Leu391位点的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Upf2 (D3B10) Rabbit mAb.

Western blot方法检测多种细胞系的提取物,使用的抗体为Upf2 (D3B10) Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Upf2 (hUpf2-Myc; +), using Upf2 (D3B10) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457.

Western blot方法检测293细胞提取物,分别用空白对照物(-)或构建的带有Myc标签的全长人Upf2 (hUpf2-Myc; +)转染细胞。使用的抗体为 Upf2 (D3B10) Rabbit mAb 兔单抗(上图) 、Myc-Tag (71D10) Rabbit mAb兔单抗 #2278(中)以及 β-Actin (D6A8) Rabbit mAb兔单抗 #8457。

Background

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).

Upf1蛋白被确认是无义介导的降解(NMD)中的一个活性组分,NMD是真核细胞中mRNA的一种监控机制,能够降解含有提前终止密码子的mRNA(1)。研究发现,Upf1蛋白是胞质中一个ATP依赖的RNA解旋酶(2),后来发现它还是细胞质加工小体(cytoplasmic P-bodies)中的一个成分(3)。Upf1蛋白的磷酸化能够介导翻译抑制,其伴随着NMD作用,因而允许mRNA更易进入NMD机制(4)。此外,还鉴定出了NMD的另外两个活化分子,Upf2和Upf3。分别定位于核周以及核质(5)。

  1. Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
  2. Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
  3. Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
  4. Isken, O. et al. (2008) Cell 133, 314-27.
  5. Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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