Cell Signaling Technology

Product Pathways - Metabolism

NBC1/SLC4A4 Antibody #11867

No. Size Price
11867S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
11867 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 135-145 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

NBC1/SLC4A4 Antibody recognizes endogenous levels of total NBC1 protein. This antibody also cross-reacts with 42 and 62 kDa proteins of unknown origin in some cell lines.

NBC1/SLC4A4 Antibody能够检测内源性NBC1总蛋白水平。在一些细胞系中该抗体可能会与某未知来源42或62kDa蛋白产生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NBC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源NBC1蛋白C末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP and LN18 cells using NBC1/SLC4A4 Antibody.

Western blot 检测 LNCaP 和 LN18细胞系提取物,使用抗体为NBC1/SLC4A4 Antibody。

IP

IP

Immunoprecipitation of NBC1/SLC4A4 from LNCaP cell extracts, using Normal Rabbit IgG #2729 (lane 2) or NBC1/SLC4A4 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NBC1/SLC4A4 Antibody.

免疫沉淀方法检测LNCaP细胞提取物NBC1/SLC4A4,使用抗体为Normal Rabbit IgG #2729 (泳道2)或NBC1/SLC4A4 Antibody (泳道3)。泳道1为10%对照。使用NBC1/SLC4A4 Antibody进行Western blot分析。

Background

NBC1/SLC4A4 is an eletrogenic sodium bicarbonate cotransporter. It mediates the coupled movement of Na+ and HCO3- ions across the basolateral membrane of cells. NBC1 regulates secretion and absorption of bicarbonate and modulates intracellular pH (1-3). There are five isoforms of NBC1 produced by alternative splicing including the most studied isoforms, 1 and 2, which have different N termini. The N terminus of isoform 1 contains multiple consensus phosphorylation sites for various kinases, such as PKA, PKC, and CK II, and may play a regulatory role in the biological function of NBC1 (4). Interaction with carbonic anhydrase II, IV, and IX regulates the transporter acitivity of NBC1 (5-7). NBC1 is expressed in various tissues with the exception of isoform 2, which is mainly expressed in kidney proximal tubules (1). Isoform 1 is expressed in the pancreas and corneal endothelium, and at low levels in other tissues including heart, skeletal muscle, liver, and prostate (4). Research studies have shown that mutations in the NBC1 gene are linked to proximal renal tubular acidosis with ocular abnormalities (also known as renal tubular acidosis II).

NBC1/SLC4A4是生电的碳酸氢钠协同转运蛋白。它介导Na +和HCO3-离子跨越细胞基底膜的耦合运动。 NBC1调节碳酸氢盐分泌和吸收,并调节细胞内pH值(1-3)。NBC1共有五种选择性剪接异构体,其中研究最多的异构体1和2,它们具有不同的N端。异构体1的N末端的包含各种激酶的多重磷酸化识别位点,如PKA,PKC,CK II,并可能对NBC1的生物学功能起到调节作用(4)。与碳酸酐酶II,IV和IX互作能调节NBC1的转运活性(5-7)。除异构体2主要是在肾近端小管表达外(1),NBC1在各种组织中都有表达。亚型1主要在胰和角膜内皮细胞表达,在其他组织,包括心脏,骨骼肌,肝脏,前列腺中表达水平较低(4)。研究表明,NBC1基因的突变与近端肾小管酸中毒(也被称为肾小管性酸中毒II)相关,该病会出现眼部异常。

  1. Burnham, C.E. et al. (1997) J Biol Chem 272, 19111-4.
  2. Amlal, H. et al. (1998) J Biol Chem 273, 16810-5.
  3. Choi, I. et al. (1999) Am J Physiol 276, C576-84.
  4. Abuladze, N. et al. (1998) J Biol Chem 273, 17689-95.
  5. Alvarez, B.V. et al. (2003) Biochemistry 42, 12321-9.
  6. Loiselle, F.B. et al. (2004) Am J Physiol Cell Physiol 286, C1423-33.
  7. Svastova, E. et al. (2012) J Biol Chem 287, 3392-402.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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