Cell Signaling Technology

Product Pathways - Protein Stability

PSMA2 (D3A4) Rabbit mAb #11864

20S Proteasome  

No. Size Price
11864S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
11864 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 25 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Bovine, Horse,

Specificity / Sensitivity

PSMA2 (D3A4) Rabbit mAb recognizes endogenous levels of total PSMA2 protein. This antibody does not cross-react with other α subunits of the 20S proteasome.

PSMA2 (D3A4) Rabbit mAb兔单抗识别内源性的总PSMA2蛋白。该抗体与20S蛋白酶体的其他α亚基无交叉反应性。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe173 of human PSMA2 protein.

该单克隆抗体经合成与人PSMA2蛋白Phe173邻近氨基酸残基序列一致的肽段,免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PSMA2 (D3A4) Rabbit mAb. Western blot检测多种细胞提取物。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMA1 (hPSMA1-Myc/DDK; +), full-length human PSMA2 (hPSMA2-Myc/DDK; +), full-length human PSMA3 (hPSMA3-Myc/DDK; +), full-length human PSMA4 (hPSMA4-Myc/DDK; +), full-length human PSMA6 (hPSMA6-Myc/DDK; +), or full-length human PSMA7 (hPSMA7-Myc/DDK; +), using PSMA2 (D3A4) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower). Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长PSMA1 (hPSMA1-Myc/DDK; +)、人全长PSMA2 (hPSMA2-Myc/DDK; +)、PSMA3 (hPSMA3-Myc/DDK; +)、PSMA4 (hPSMA4-Myc/DDK; +)、PSMA6 (hPSMA6-Myc/DDK; +)、PSMA7 (hPSMA7-Myc/DDK; +)的293T细胞,采用PSMA2 (D3A4) Rabbit mAb (upper) 或DYKDDDDK Tag Antibody #2368 (lower)。

Background

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

20S蛋白酶体是参与细胞内蛋白降解的一种主要的蛋白水解酶复合物。它包括4个堆积的环状结构,每一个环状结构具有7个不同的亚基。2个外层是相同的环状结构,由α 亚基(又称为PSMAs)组成,2个内层由β亚基组成的相同的环状结构。尽管催化位点定位于β环(1-3),α亚基对于组装具有重要作用,由于其具有调节蛋白的结合位点(4)。7种不同的α 和10种不同的β蛋白酶体基因已经在哺乳动物中被确定(5)。PA700,PA28和PA200是3种主要的蛋白复合物,主要作为20S蛋白酶体PA700结合具有高亲和力泛素的激活因子,并与20S蛋白酶体形成优先降解泛素化蛋白26S蛋白酶体有关(1-3)。蛋白酶体具有广泛的底物谱,包括细胞周期调节因子,信号分子,肿瘤抑制因子以及转录因子。通过控制这些细胞内蛋白的降解,蛋白酶体在细胞周期调节、癌症发生、免疫应答、蛋白折叠以及疾病进展中均发挥作用(6-9)。

  1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48.
  2. Pickart, C.M. and Cohen, R.E. (2004) Nat. Rev. Mol. Cell Biol. 5, 177-187.
  3. Nandi, D. et al. (2006) J. Biosci. 31, 137-155.
  4. Lupas, A. et al. (1993) Enzyme Protein 47, 252-273.
  5. Monaco, J.J. and Nandi, D. (1995) Annu. Rev. Genet. 29, 729-754.
  6. Murray, A.W. (2004) Cell 116, 221-234.
  7. Ciechanover, A. (2006) Proc. Am. Thorac. Soc. 3, 21-31.
  8. Wang, J. and Maldonado, M.A. (2006) Cell. Mol. Immunol. 3, 255-261.
  9. Rubinsztein, D.C. (2006) Nature 443, 780-786.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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