Cell Signaling Technology

Product Pathways - Protein Stability

USP8 (D18F6) Rabbit mAb #11832

No. Size Price
11832S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
11832 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 140 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

USP8 (D18F6) Rabbit mAb recognizes endogenous levels of total USP8 protein.

USP8 (D18F6) Rabbit mAb兔单抗识别内源性的总USP8蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu614 of human USP8 protein.

此单克隆抗体经合成与人USP8蛋白Leu614邻近氨基酸残基序列一致的肽段,免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP8 (D18F6) Rabbit mAb.Western blot检测多种细胞提取物,采用USP8 (D18F6) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human USP8 (hUSP8-Myc/DDK; +), using USP8 (D18F6) Rabbit mAb.Western blot检测mock transfected (-) 或者转染表达Myc/DDK-tagged人全长USP8 (hUSP8-Myc/DDK; +)的293T细胞,采用USP8 (D18F6) Rabbit mAb。

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival (3,4). Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met (4). In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR (5,6). Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination (7). It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation (8,9).

泛素化作用酶(UBEs)催化蛋白的泛素化,与去泛素化作用酶(DUB)的过程可逆(1,2)。5种DUB亚家族已经被确定,包括USP, UCH, OTU, MJD和JAMM酶。去泛素酶泛素特异蛋白酶8(USP8/UBPy)是一种半胱氨酸蛋白酶,属于USP/UBP亚家族。研究表明USP8对于细胞增殖和存活是一种必要的生长调节酶(3,4)。确实,USP8的敲除小鼠被证实可以引起酪氨酸激酶受体表达的显著缺失,这些受体包括EGFR, ErbB3和 c-Met (4)。与这些发现一致,USP8失活导致配体活化EGFR去泛素化水平加强(5,6)。此外,USP8在Ser680磷酸化导致其与14-3-3的结合,催化活性和降低EGFR去泛素化作用(7)。尽管USP8与ESCRT-0复合物的组分相结合,在早期选择性内化机制中起整合作用,该机制可以保护EGFR免于溶酶体的降解(8,9)。

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
  4. Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
  5. Alwan, H.A. and van Leeuwen, J.E. (2007) J Biol Chem 282, 1658-69.
  6. Mizuno, E. et al. (2005) Mol Biol Cell 16, 5163-74.
  7. Mizuno, E. et al. (2007) Exp Cell Res 313, 3624-34.
  8. Row, P.E. et al. (2006) J Biol Chem 281, 12618-24.
  9. Berlin, I. et al. (2010) J Biol Chem 285, 34909-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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