Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TCF12/HEB (D2C10) Rabbit mAb #11825

No. Size Price
11825S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11825 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 85 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Horse,

Specificity / Sensitivity

TCF12/HEB (D2C10) Rabbit mAb recognizes endogenous levels of total TCF12/HEB protein. This antibody recognizes the TCF12/HEBCan isoform, but not the TCF12/HEBAlt isoform.

TCF12/HEB (D2C10)Rabbit mAb兔单抗可以识别内源性的总TCF12/HEB蛋白。抗体可以识别TCF12/HEBCan 亚型,但是无法识别TCF12/HEBAlt亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly115 of human TCF12 protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人TCF12蛋白Gly115邻近氨基酸残基一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and MOLT-4 cells using TCF12/HEB (D2C10) Rabbit mAb.

使用TCF12/HEB (D2C10)Rabbit mAb兔单抗对Jurkat和MOLT-4细胞提取物进行western blot分析。

Background

TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The TCF12/HEB gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5).

TCF12/HEB(T细胞因子12/HeLa E-box结合蛋白)是一种E蛋白,是一种含有I型碱性螺旋-环-螺旋结构域的转录因子,直接和E-box DNA结合,控制多种发育过程(1,2)。E蛋白家族成员成员包括E2A, E2-2,和TCF12/HEB. TCF12/HEB会与其他E蛋白形成同源或异源二聚体调控目的基因的转录。在T细胞,B细胞和树突状细胞发育过程中发挥重要作用(2-4)。TCF12/HEB基因有2个转录起始位点导致会形成一个较长的亚型称为HEBCan,以及较短的亚型HEBAlt(5)。这两个亚型被认为有不同的功能,HEBCan在T细胞发育过程发挥作用,而HEBAlt在T祖细胞形成过程中发挥作用(5)。

  1. Murre, C. (2005) Nat Immunol 6, 1079-86.
  2. Braunstein, M. and Anderson, M.K. (2012) Clin Dev Immunol 2012, 678705.
  3. Quong, M.W. et al. (2002) Annu Rev Immunol 20, 301-22.
  4. Kee, B.L. (2009) Nat Rev Immunol 9, 175-84.
  5. Wang, D. et al. (2006) J Immunol 177, 109-19.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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