Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

DEPTOR/DEPDC6 (D9F5) Rabbit mAb #11816

DEP domain containing 6   DEP domain containing mTOR interacting protein   DEP domain-containing mTOR-interacting protein   DEP domain-containing protein 6   DEP.6   DEPD6   DEPDC6   Deptor   DKFZp564B1778   FLJ12341   FLJ12428   FLJ13854  

No. Size Price
11816S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
11816 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 48 Rabbit IgG
IP 1:50
IHC-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

DEPTOR/DEPDC6 (D9F5) Rabbit mAb recognizes endogenous levels of total DEPTOR/DEPDC6 protein.

DEPTOR/DEPDC6 (D9F5)Rabbit mAb兔单抗可以识别内源性总的DEPTOR/DEPDC6 (D9F5)蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human thyroid using DEPTOR/DEPDC6 (D9F5) Rabbit mAb.

使用DEPTOR/DEPDC6 (D9F5) Rabbit mAb兔单抗对石蜡包埋的人甲状腺组织进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from RPMI 8226, MCF7, and INS-1 cells using DEPTOR/DEPDC6 (D9F5) Rabbit mAb.

使用DEPTOR/DEPDC6 (D9F5) Rabbit mA兔单抗对RPMI 8226, MCF7和 INS-1细胞提取物进行western blot分析。


DEPTOR/DEPDC6 is a component of both mTORC1 and mTORC2 complexes (1). It interacts with mTOR and inhibits mTORC1 and mTORC2 kinase activities (1). mTOR, along with casein kinase I, phosphorylates DEPTOR/DEPDC6 in the presence of growth signals, which leads to the degradation of DEPTOR/DEPDC6 (2). Research studies have shown that transgenic mice overexpressing DEPTOR/DEPDC6 have more white adipose tissue, and DEPTOR/DEPDC6 expression levels increase in the white adipose tissue of obese humans (3). Furthermore, the expression of DEPTOR/DEPDC6 is induced during mouse adipocyte differentiation (3). Together these findings suggest that DEPTOR/DEPDC6 regulates adipogenesis (3).


  1. Peterson, T.R. et al. (2009) Cell 137, 873-86.
  2. Gao, D. et al. (2011) Mol Cell 44, 290-303.
  3. Laplante, M. et al. (2012) Cell Metab 16, 202-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :