Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRAK1 (D51G7) Rabbit mAb (Biotinylated) #11123

No. Size Price
11123S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
11123 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 78-105 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total IRAK1 protein.

IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated)能够检测内源IRAK1蛋白总体水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRAK1 protein.

此单克隆抗体是通过合成鼠源对应的IRAK1 C-末端周围的肽段来免疫动物而获得。

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated IRAK1 (D51G7) XP® Rabbit mAb #4504.

CST公司生产的此抗体与生物素在特定的条件下偶联。正如预期,生物素偶联的抗体与未偶联的抗体 IRAK1 (D51G7) XP® Rabbit mAb #4504相同,都能够与同物种中的蛋白交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.Western免疫印迹。用IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated)研究各种细胞系的细胞提取液。Streptavidin-HRP #3999 用于检测。

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).

细胞白介素1(IL-1)受体相关激酶(IRAK)是丝氨酸/酪氨酸特异性激酶,它通过IL-1诱导的方式与IL-1受体发生共沉淀(1)。 哺乳动物的IRAK家族有四个成员(IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4)。IL-1结合到IL-1的I类受体(IL-1RI)而促进形成包含IL-1RI, AcP, MyD88和IRAKs的复合体(2)。IRAK在IL-1刺激后能够在短时间内自磷酸化。接着IRAK从IL-1RI复合体上解离,泛素化并与两个膜结合蛋白 TAB2 和TRAF6结合。IRAK-TRAF6-TAB2复合体释放到细胞质中并激活蛋白激酶的级联反应,包括TAK1、IKKs 和应激活化激酶 (3)。

  1. Dinarello, C.A. (1996) Blood 87, 2095-147.
  2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
  3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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