Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD81 (D5O2Q) Rabbit mAb (Mouse Specific) #10037

No. Size Price
10037S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
10037 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 22 Rabbit IgG
IP 1:50
F 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CD81 (D5O2Q) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total CD81 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro184 of mouse CD81 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of EL4 cells (blue) and Neuro-2A cells (green) using CD81 (D5O2Q) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 (positive, left) and BaF3 (negative, right) cells using CD81 (D5O2Q) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CD81 (D5O2Q) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD81 (mCD81-Myc/DDK, +), using CD81 (D5O2Q) Rabbit mAb (Mouse Specific).

IP

IP

Immunoprecipitation of CD81 from extracts of C2C12 cells. Lane 1 is 10% input, Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD81 (D5O2Q) Rabbit mAb (Mouse Specific). Western blot analysis was performed using CD81 (D5O2Q) Rabbit mAb (Mouse Specific).

Background

CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8, 9).

  1. Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15.
  2. Levy, S. et al. (1991) J Biol Chem 266, 14597-602.
  3. Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
  4. Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7.
  5. Levy, S. et al. (1998) Annu Rev Immunol 16, 89-109.
  6. Levy, S. and Shoham, T. (2005) Nat Rev Immunol 5, 136-48.
  7. Jones, E.L. et al. (2011) Biochem Soc Trans 39, 506-11.
  8. Pileri, P. et al. (1998) Science 282, 938-41.
  9. Bartosch, B. et al. (2003) J Biol Chem 278, 41624-30.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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